Journal: Glycobiology
Article Title: A synthetic HS structure selectively impairs the morphology and function of excitatory synapse by disrupting neurexin1 interactions
doi: 10.1093/glycob/cwaf039
Figure Lengend Snippet: Effects of 12-mer-19 (19) and 12-mer-NAc (NAc) on the function excitatory synapses (A-C) and inhibitory synapses (D-E). A) Traces of mEPSC recordings from primary rat hippocampal neurons. B) Treatment of 12-mer-19 resulted in a reduction in mEPSC frequency. There was no significant change between control (Con) and 12-mer-NAc-treated groups. C) There was a significant decrease in EPSC amplitude between 12-mer-19 and control groups but not between 12-mer-19 and 12-mer-NAc groups. D) Traces of mIPSC recordings from primary rat hippocampal neurons. E and F) No significant difference was observed among all groups in the frequency (E) and amplitude (F) of mIPSC. ****p<0.0001 or *p=0.01 by one-way ANOVA with posthoc Bonferroni’s multiple comparison tests. N=21-24 cells from 6 independent experiments for mEPSC. N=19-22 cells from 3 independent experiments for mIPSC. Error bars represent SEM.
Article Snippet: Primary rat hippocampal neuron cultures were prepared from embryonic day 18 rat (Charles River, CD 001) using the Banker-style culture method ( Kaech and Banker 2006 ).
Techniques: Control, Comparison